Cells attached to cell culture flasks starting to detach, one after the other, then, with a gush of cell culture medium the cells are afloat.

Trypsinization – the State of High Vulnerability 💥

During trypsinization, cells are most vulnerable. Enzymes ✂ nibble off their anchorage to safe plastic. Bad enough. But once the anchorage is gone, enzymes turn their attention to their next victims, the cells themselves 😳!

If you keep cells in trypsin for too long, or if the trypsin concentration is not right, the cells themselves may fall prey to the enzyme’s gluttony 😮.

But there are other 🫣, less obvious factors that pose a threat to cells during trypsinization ☝.

Do you know them?

 

#Trypsinization #CellCulture #GCCP #LabLife

Author: asban