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Welcome to our complete package of video tutorials on cell culture technique. All commonly used procedures in the cell culture lab are described and discussed here.

Content

We not only follow the rules for Good Cell Culture Practice, we also show, in great detail, how good practices should actually be implemented. Topics of the tutorials start from how to get ready for the cell culture lab, then we talk about cells, and everything connected to cells, such as cell culture medium, cell morphology, cell confluency, cell types, and cell line misidentification.

There is also an entire section dedicated to different kinds of vessels such as tubes, but also for the cultivation of cells, such as flasks and plates. We discuss passaging, counting and freezing cells, and we talk about contaminations in the cell culture lab. Of course, we also talk about lab equipment, in particular about pipetting with various pipettes. Ultimately, vessels, equipment and the way we treat our cells have a direct effect on them and on the data we generate.

The tutorials include common mistakes, common misconceptions, food for thought, but also good habits, opinions, tips and hacks, and do’s and don’ts.

Who will benefit

The tutorials can be watched in class, after class or before starting a practical course, or before starting a bachelor, a master or a PhD programme. They can be watched before starting to work in a cell culture lab, or before switching to a different workplace that happens to be a cell culture lab.

We hope the videos encourage you to discuss why things are done a certain way in the cell culture lab, and where things can still be improved. Regarding the order of the videos, we recommend a certain order, but, of course, you are free to choose a different order if that makes more sense to you and your situation.

Course Content

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Getting ready
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Cells
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