Are you ready to enter the cell culture lab? Have you thought of everything? With good preparation, you can already set the course for your experiments later. Here is a checklist for before you enter and before you leave the cell culture lab. Please adjust this checklist to your own situation, if needed.
Everything you do in the cell culture lab is aimed at a successful experiment. This makes it all the more important to plan each experiment well and carry it out in the best possible way. We do not want to waste time, energy and resources, if we can avoid it. Complete this checklist with points that are important for your own situation.
Some of us panic when we suspect a contaminant roaming our culture. Don’t let this happen to you, it doesn’t help. Find out whether you really have a contamination, and then follow the protocol of what to do in such a situation. With this Learning Toolbox you can find out, if your unidentified floating object (“UFO”) in your culture could really be a contaminant.
Here, we calculate the molar concentration of two different substances. One is MgSO4 anhydrous (MgSO4) and the other contains water (MgSO4.7H2O). Do you have to incorporate the 7 H2O into your calculation, and if so, how do you do that?
Authors: David Pamies, Marcel Leist, Sandra Coecke, Gerard Bowe, David G. Allen, Gerhard Gstraunthaler, Anna Bal-Price, Francesca Pistollato, Rob B. M. de Vries, Helena T. Hogberg, Thomas Hartung and Glyn Stacey.
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