This is a step-by-step guide on how to change the medium of a cell culture flask. You will find a lot of tips and reminders for this routine procedure in the cell culture lab.
Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)
Journal: ALTEX 39(1), 030-070.doi:10.14573/altex.2111011
Authors: David Pamies, Marcel Leist, Sandra Coecke, Gerard Bowe, David G. Allen, Gerhard Gstraunthaler, Anna Bal-Price, Francesca Pistollato, Rob B. M. de Vries, Helena T. Hogberg, Thomas Hartung and Glyn Stacey.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0).